Intestinal Permeability Testing Methods: What Each Test Can (and Can't) Tell You

D2 • Feb 28, 2026 • 9 min read

A practical comparison of permeability testing methods and how to interpret them without overreach.

Quick answer

No single permeability test is perfect. The practical approach is to use the right test for the question and interpret results in context.

Main options

  • Lactulose-mannitol: functional barrier signal useful for trend tracking.
  • Zonulin: indirect marker that should not be used as a standalone diagnosis.
  • Rule-out testing: celiac/inflammatory workup when clinically indicated.

Bottom line: testing helps when it drives decisions, not when it becomes a label.